Cryoprotectant delivery and removal from murine insulinomas at vitrification-relevant concentrations
نویسندگان
چکیده
منابع مشابه
L-proline: a highly effective cryoprotectant for mouse oocyte vitrification
Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the...
متن کاملOptimization of cryoprotectant loading into murine and human oocytes.
Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity. Thus, we investigated the use of physics-based mathematical optimization to guide design of cryoprotectant loading methods for mouse and human oocytes. We first examined loading of 1.5 M dimethyl sulfoxide (M...
متن کاملEffects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions
Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. We report systematic measurements of critical cryoprotective agent (CPA) concentrations required for vitrification during plunge cooling from T=295 K to T=77 K in liquid nitrogen. Measurements on fourteen common CPAs including alcohols (glycerol, methanol, isopropanol), su...
متن کاملLarge volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa.
Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free vitrification to conventional cryopre...
متن کاملRate of disappearance of endogenous insulin from the circulation after removal of insulinomas.
In two patients with insulinomas a rapid rate of disappearance of insulin from the circulation was found after the tumours had been removed. In a third case the rate of disappearance was slower but the insulin was not immunologically identical to normal human insulin standards, as it was in the other two cases.
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ژورنال
عنوان ژورنال: Cryobiology
سال: 2007
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2007.04.002